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. Author manuscript; available in PMC: 2010 Jun 2.
Published in final edited form as: Cancer Cell. 2009 Jun 2;15(6):501–513. doi: 10.1016/j.ccr.2009.03.018

Figure 2. Glioma Stem Cells and Normal Neural Progenitors Differentially Expressed Hypoxia Response Genes.

Figure 2

Cells from T3565 and T4302 glioblastoma samples and two different normal neural progenitor cell preparations were cultured in normoxia (20% oxygen) or hypoxia (1% oxygen) for 24 hr. RT-PCR analysis was performed with primers specific for HIF2α (A), HIF1α (B), Oct4 (C), PGK1 (D), Glut1 (E), TGF-α (F), SerpinB9 (G), and VEGF (H). Data were normalized to β-actin levels. *, P < 0.01 with ANOVA comparison of hypoxia treated cells to identically prepared normoxia controls. #, P < 0.01 with ANOVA comparison of indicated hypoxia treated cells to both hypoxia treated GSCs.