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. Author manuscript; available in PMC: 2010 Jun 2.
Published in final edited form as: Cancer Cell. 2009 Jun 2;15(6):501–513. doi: 10.1016/j.ccr.2009.03.018

Figure 7. HIF Knockdown Decreased Glioma Stem Cell Mediated Angiogenesis.

Figure 7

(A) HIF2α knockdown prevents hypoxia induced activation of the VEGF Promoter. *, P < 0.001 with ANOVA comparison of HIF2α or HIF1α shRNA treated stem cells to nontargeting shRNA with hypoxia. (B) HIF knockdown reduces VEGF protein expression in GSCs. (C) HIF2α knockdown reduces VEGF expression in stem, but not nonstem, glioblastoma cells. *, P < 0.01 (D-E) HIF knockdown reduces VEGF mRNA level in GSCs. *, P < 0.01 (F-H) HIF2α knockdown in GSCs reduced cancer cell-mediated endothelial cell proliferation. (F) Representative diagram of the coculture assay. (G) Representative images of cocultured HMVEC cells after cells fixed with 4% PFA and then stained with toluidine blue. (H) HMVEC proliferation was measured through [3H]-thymidine incorporation. *, P < 0.001 by ANOVA with comparison of HIF2α or HIF1α shRNA infected glioma cells to corresponding nontargeting shRNA infected cells. #, P < 0.001 by ANOVA comparison of nontargeting shRNA infected GSCs to nonstem nontargeting control cells.