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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Biotechniques. 2009 Feb;46(2):115–126. doi: 10.2144/000113068

Figure 3. Real-time RT-PCR for miR-150 precursors and mature molecules in two cancer cell lines.

Figure 3

Real-time RT-PCR shows that miR-150 precursors were each present in the cell lines HL60 and Jurkat (A), while only the mature form of miR-150 was detectable in the Jurkat cell line (B). The cell fractionation experiments showed that the mature miR-150 was present in much higher amounts in the cytoplasm (black bar) than the nucleus (gray bar) in the Jurkat cells (C) and, thus, was consistent with the ultramer extension data (see Figure 4).