Table 1.
Effect of inhibition of PAO by MDL72527 on pancreatic SSAT activity and polyamine concentrations in metallothionein-SSAT rats treated with zinc
| Group | SSAT, pmol/mg/10 min | Putrescine | N1-Acetylspermidine, pmol/mg tissue | Spermidine | N1-Acetylspermine | Spermine |
|---|---|---|---|---|---|---|
| Sg | 5.5 ± 2.6 | 23 ± 20 | ND | 3,990 ± 330 | ND | 560 ± 50 |
| Sg + MDL | 7.2 ± 1.0 | 22 ± 19 | ND | 4,080 ± 580 | ND | 800 ± 110 |
| Sg + Zn | 3.7 ± 0.6 | 36 ± 27 | ND | 3,620 ± 210 | ND | 480 ± 50 |
| Sg + MDL + Zn | 3.1 ± 0.6 | 29 ± 20 | 61 ± 18*** | 3,740 ± 640 | ND | 700 ± 130 |
| Tg | 37.5 ± 4.8 | 2,950 ± 1,620 | 33 ± 10 | 2,630 ± 190 | ND | 500 ± 40 |
| Tg + MDL | 48.0 ± 13.0 | 1,340 ± 220 | 2,090 ± 390*** | 2,740 ± 220 | ND | 420 ± 40 |
| Tg + Zn | 636 ± 347* | 2,830 ± 760 | 41 ± 9 | 370 ± 150*** | ND | 64 ± 36*** |
| Tg + MDL + Zn | 310 ± 50*** | 780 ± 140* | 1,340 ± 100*** | 320 ± 100*** | 37 ± 5* | 120 ± 50*** |
Combined treatment: rats were injected i.p. with 30 mg/kg of MDL72527 and 1 h later with 10 mg/kg of zinc. Animals were killed 24 h after the zinc injection. There were three to four animals in each group. *, P < 0.05; ***, P < 0.001 as compared with untreated animals. Sg, syngenic; Tg, transgenic; ND, not detected.