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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Virology. 2007 Oct 24;370(1):1–11. doi: 10.1016/j.virol.2007.08.003

Table 4.

Differential levels of gene expression as determined by DNA microarray and real time RT-PCR1

Gene Symbol Gene Title DNA microarray Real time RT-PCR
Gene expression in RUB-infected HEF compared to mock-infected HEF
APAF1 Apoptotic peptidase activating factor −3.222 −1.302*
CASP1 Caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) 15.37 6.63
CFLAR CASP8 and FADD-like apoptosis regulator 2.68 1.38
DAPK1 Death-associated protein kinase 1 −2.32 −5.59
RIPK2 Receptor-interacting serine-threonine kinase 2 2.13 1.76*
Gene expression in RUB-infected Hs888Lu compared to mock-infected Hs888Lu
CFLAR CASP8 and FADD-like apoptosis regulator 2.09 2.07
Gene expression in HEF compared to Hs888Lu
BCL2L10 BCL2-like 10 3.07 4.54
CASP1 Caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) −9.87 −4.72
CASP4 Caspase 4, apoptosis-related cysteine peptidase −2.93 −2.17
CASP8 Caspase 8, apoptosis-related cysteine peptidase −2.74 −6.44
LTBR Lymphotoxin beta receptor (TNFR superfamily, member 3) −4.3 −1.62*
TNFRSF11B Tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) −11.42 −6.96
1

The indicated pro- and apoptotic genes found to be up- or down-regulated by microarray (>2 fold) were also analyzed using a quantitative RT-PCR assay to confirm the level of up- or down-regulation.

2

Negative values indicate downregulation.

*

Regulation of < 2 fold that were statistically significant (p values < 0.05)