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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Traffic. 2008 Nov 18;10(2):201–217. doi: 10.1111/j.1600-0854.2008.00856.x

Figure 5. STAM2 depletion results in a highly condensed Golgi apparatus.

Figure 5

A) Lysates from HeLa cells transfected with either of two different siRNAs specific for STAM2 or else control siRNA were immunoblotted for STAM2 at 72 h post-transfection. Equal protein loading was monitored by immunoblotting for PLCγ. STAM1 levels are unchanged. B) Immunostaining of HeLa cells for STAM2 after transfection with control siRNA or STAM2 siRNA #1. Bar, 10 μm. C) HeLa cells transfected with control siRNA or STAM2 siRNA #1 were immunostained for GM130. STAM2 siRNA cells have a highly condensed Golgi apparatus and clustered ERES. Bar, 10 μm. D) Graphical representation of percentage of cells with Golgi condensation in STAM2 siRNA and control siRNA cells 72 h after transfection (n=3; 100 cells per condition; ±SD). *p <0.001. E) Graphical representation of the ratio of Golgi circumference to cell circumference in control versus STAM2 siRNA cells (n=3; 100 cells per condition). **p=0.02. F) Electron microscopy images of the Golgi complex in control cells (single asterisk; left panel) compared with that in STAM2 siRNA cells, where multiple Golgi cisternae are seen at the same magnification (asterisks; right panel). Bar, 500 nm.