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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Traffic. 2008 Nov 18;10(2):201–217. doi: 10.1111/j.1600-0854.2008.00856.x

Figure 6. Knock down and overexpression of STAM1 confirms some functional redundancy with STAM2.

Figure 6

A) Left panel, Extracts from HeLa cells transfected with any of three siRNA oligonucleotides specific for STAM1 or else with control siRNA were immunoblotted at 72 h post-transfection. Levels of STAM2 were unaltered. Right panel, Extracts from HeLa cells transfected with control siRNA or cotransfected with siRNAs for both STAM1 and STAM2 (STAM DKD) were immunoblotted with the indicated antibodies. Equal protein loading was monitored using PLCγ. B) HeLa cells were transfected with STAM1 siRNA or co-transfected with siRNAs for STAM1 and STAM2 (STAM double knock down [DKD]) or else with control siRNA. Percentages of cells with Golgi condensation (≤5 μm diameter in all dimensions) in STAM1 siRNA, STAM DKD, and control siRNA groups (n=3; 100 cells per experiment) are presented graphically (±SD). *p=0.0042; **p<0.0001. C) HeLa cells transfected with control, STAM1, or STAM DKD siRNAs were immunostained with anti-GM130 antibodies. As observed for STAM2 siRNA knock down cells, STAM1 siRNA cells also have a highly condensed Golgi apparatus. Bar, 10 μm. D) Re-transfection of HeLa cells with Myc-STAM1 after transfection with STAM2 siRNA suppressed the condensed Golgi phenotype (arrow). Bar, 10 μm.