FIG. 2.

H134R-ChR2 generates excitatory junctional potentials (EJPs) at the larval neuromuscular junction more effectively than ChR2. A: schematic of neuromuscular junction (NMJ) preparation showing sharp electrode and targeted muscle (m6). Brain and posterior-most nerves are cut to silence crawling rhythms. B: light-evoked EJPs in response to 200-ms light pulses at various intensities in an OK371-GAL4/UAS-ChR2 and an OK371-GAL4/UAS-H134R-ChR2 preparation. C: EJP frequency (means ± SE) is shown as a function of light intensity in cells expressing ChR2 and H134R-ChR2 (200 ms pulses, 470 nm). P < 0.005 for all comparisons (Student's t-test).