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Thresholds for evoking single EJPs at the m6 neuromuscular junction are different for ChR2 and H134R-ChR2. A: single EJPs evoked by 10-ms pulses of varying intensity. Blue arrows denote light pulses. Note that EJPs are evoked at a lower light intensity in the H134R-ChR2–expressing preparation. B: percent of responses as a function of light intensity and duration in the 2 ChR2 variants. Pooled data shown as means ± SE. *P < 0.05, Student's t-test.
