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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):2868–2878. doi: 10.4049/jimmunol.0802368

Figure 9.

Figure 9

Deficient transcriptional activation at the A1 and c-Myc promoter in T1 B cells upon BCR crosslinking. A–B, Chromatin from WT T1 and FM B cells stimulated 60 or 90 min with anti-IgM was immunoprecipitated with antibodies that recognize Pol II or polyclonal rabbit IgG as a control. PCR was performed on DNA isolated from immunoprecipitates or 5% of input DNA (loading control) to amplify a DNA region within the A, A1 promoter, or B, c-Myc promoter. Shown is a representative figure of 3 independent experiments (left panel) and mean with SD of the fold increase in PCR product over unstimulated cells (right panel). C–E, Chromatin from WT T1 and FM B cells stimulated 90 min with anti-IgM or CpG was immunoprecipitated as above and PCR was performed as indicated. C, Representative figure of 4 independent experiments. D–E, Mean with SD of the fold increase in PCR product over unstimulated cells obtained using primers recognizing the D, A1 promoter, or E, c-Myc promoter.