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. 2009 May 22;9:15. doi: 10.1186/1472-6882-9-15

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Copyright © 2009 Lopes et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of Pt in HUVEC proliferation evaluated by BrdU assay. Incubation with 8 μM Pt resulted in a very significant decrease in the percentage of proliferating cells. Bars represent the percentage of proliferating cells evaluated by the ratio of BrdU stained cells in 1000 hematoxylin stained nuclei. Assays were repeated five times and performed in triplicate. Statistical differences between groups were evaluated by one-way analysis of variance (ANOVA test) followed by the Dunnet test. *p < 0.01 vs Control.