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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Apr 11;49(8):3622–3630. doi: 10.1167/iovs.07-1559

Figure 2. Oxidative stress results in an accumulation of ubiquitin conjugates.

Figure 2

(A) ARPE-19 cells were exposed to 10 or 50 μM H2O2 for 4 hours and levels of endogenous ubiquitin conjugates were determined by Western blotting. (B) ARPE-19 cells were treated with blue light alone, A2E alone, or both A2E and blue light as described. Levels of endogenous ubiquitin conjugates were determined by Western blotting. Levels of β-actin were used as protein loading controls.