A, B and C: The expressions of Vcsa1, AR and Slo in the corpora of the same experimental groups described in Figure 1 are shown. The number of animals in each group was as follows; control group N=3 animals, orchiectomized N=3 animals and orchiectomized plus testosterone, N=6 animals. Expression of transcripts were normalized to GAPDH and analyzed using the comparative crossing threshold (Ct) method. The control group corporal tissue was used as the calibrator tissue (set as 1). Each quantitative RT-PCR measurement was performed in duplicate for each sample. The bars represent the mean comparative expression of the gene, and the error bars the standard deviation. *=Significantly different expression of Vcsa1, AR or Slo in the corpora of orchiectomized compared to control animals (P < 0.05), **=Significantly different expression of transcripts in the corpora of orchiectomized treated with testosterone compared to the orchiectomized group of animals, (P < 0.05).