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CE analysis of the (A) crude one-pot reaction of WbpA, WbpB, WbpE, WbpD and WbpI and (B) purified UDP-ManNAc(3NAc)A, with both NAD+ and UDP-GlcNAc(3NAc)A included as references. UDP-ManNAc(3NAc)A has the same retention time as NADH. (1) NAD+; (2) UDP-GlcNAc; (3) AcCoA; (4) NADH; (5) UDP-ManNAc(3NAc)A; (6) CoA; (7) UDP-GlcNAc(3NAc)A.
