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Identification of NAD+ as the bound cofactor to WbpB by UV analysis. WbpB extract was treated with either NAD-dependent alcohol dehydrogenase (AD) or NADP-dependent isocitrate dehydrogrenase (ID) and analyzed for the formation of NADH/NADPH by absorbance at 340 nm. (1) Enzyme added to extract in cuvette; (2) NADP+ (AD) or NAD+ (ID) added to reaction to address specificity; (3) NAD+ (AD) or NADP+ (ID) added to reaction to check for activity.
