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Michaelis-Menten diagram depicting WbpD kinetic parameters. Reactions were carried out 50 mM HEPES, pH 7.0 at 30 °C using 0.5 ng WbpD, with AcCoA as the fixed substrate (1.5 mM) and UDP-GlcNAc(3NH2)A as the variable substrate (0.015-1.5 mM).
