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. Author manuscript; available in PMC: 2010 Jun 20.
Published in final edited form as: Virology. 2009 May 14;389(1-2):45–58. doi: 10.1016/j.virol.2009.03.012

Figure 5. Luciferase assay of coxsackievirus IRES with poly(G) substitutions in stem-loop IV.

Figure 5

A.Luciferase reporter RNAs (50 fmol) containing the poliovirus 5′ NCR, coxsackievirus 5′ NCR, poly(G)-loop coxsackievirus 5′NCR, or poly(G)-bulge coxsackievirus 5′ NCR were incubated in HeLa S10 cytoplasmic extracts for 2.5 h at 30°C and then assayed for luciferase. The relative light unit (RLU) values are averages from three separate experiments.