Fig. 3.

Immunoblots (A-C) and immunoblot data summaries (D) for ENaC subunits. Representative blots are from 40 week-old LETO and OLETF rats. Each lane was loaded with whole kidney homogenate from a different rat. Equal amounts of protein were loaded in all lanes. Immunoblots were probed with (A) anti-ENaC α-subunit (3560-2-4), (B) anti-ENaC β-subunit (3755-2), and (C) anti-ENaC γ-subunit (A881) antibodies. Band densities of the ENaC β-subunit were significantly increased, and band densities for the ENaC γ-subunit were significantly decreased in OLETF rats vs. LETO rats. The relative abundances of ENaC α-subunit were not affected by rat type. ^*p<0.05