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. 2009 Jun 26;5(6):e1000532. doi: 10.1371/journal.pgen.1000532

Figure 1. Nec is detected in shits1 garland cells.

Figure 1

(A) Wild-type garland cells (white arrow) 6 h post infection. (B) shits mutant 6 h post infection showing strong Nec localisation (red) to garland cells (white arrow). (C) Wild-type garland cells (white arrow) without infection. (D) shits mutant garland cells (white arrow) without infection show faint, basal Nec levels. The ring of garland cells is loosely inter-connected with fine Actin fibres, which frequently break during preparation. All preparations were fixed at 37°C. Nec (red), Actin (green), and DAPI (DNA) (blue).