Skip to main content
. 2009 Jun 16;4(6):e5936. doi: 10.1371/journal.pone.0005936

Figure 5. In situ hybridization (ISH) analysis of control and bulgy morph tadpoles.

Figure 5

(a) Tadpoles were longitudinally sectioned (5 µm) at the frontal side (1), the ventral side (2), and the dorsal side (3). (b) ISH analysis of bulgy tadpoles with an anti-sense probe for type-1 collagen expression. Arrowhead indicates type-1 collagen signal. (c) ISH of bulgy tadpoles with an anti-sense probe for pirica expression. Arrowhead indicates the signal for pirica expression. (d) Pirica gene expression with an anti-sense probe at the frontal side (1), ventral side (2), and dorsal side (3) of a bulgy morph tadpole. (e) ISH of pirica gene expression using a sense probe. (f) ISH of pirica gene expression in a control tadpole using an anti-sense probe. (g) ISH of pirica gene expression in a control tadpole using a sense probe.