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. 2009 Jun 17;4(6):e5949. doi: 10.1371/journal.pone.0005949

Figure 6. Hsp90 can attenuate CHIP-mediated LRRK2 degradation.

Figure 6

(A) 1.0 µg pMyc-LRRK2 and 0.5 µg pHA-CHIP were co-transfected with 0, 0.1, 0.4, 0.6, 1.0 µg pFlag-Hsp90. After 48-hour incubation, the cell lysates were immunoblotted with anti-myc and anti-β-actin antibodies. (B) 1.0 µg of pMyc-LRRK2 was co-transfected with 0.5 pHA-CMV (Lane 1 and 2 from left to right) or 0.5 µg pHA-CHIP (Lane 3–8). 24 hours after cell transfection, GA and lactacystin were added to the indicated concentrations and the cells were cultured for another 24 hours prior to cell collection and western analysis.