Table II.

Sequences of primers and probes used for Taqman RT-PCR analysis

Cyclooxygenase-1 (COX-1)	Forward Reverse Probe	5′-TGT TCG GTG TCC AGT TCC AAT A-3′ 5′-ACC TTG AAG GAG TCA GGC ATG AG-3′ 5′-CGC AAC CGC ATT GCC ATG GAG T-3′
Cyclooxygenase-2 (COX-2)	Forward Reverse Probe	5′-CCT TCC TCC TGT GCC TGA TG-3′ 5′-ACA ATC TCA TTT GAA TCA GGA AGC T-3′ 5′-TGC CCG ACT CCC TTG GGT GTC A-3′
Prostaglandin E2 receptor type 1 (EP1)	Forward Reverse Probe	5′-AGA TGG TGG GCC AGC TTG T-3′ 5′-GCC ACC AAC ACC AGC ATT G-3′ 5′-CAG CAG ATG CAC GAC ACC ACC ATG-3′
Prostaglandin E2 receptor type 2 (EP2)	Forward Reverse Probe	5′-GAC CGC TTA CCT GCA GCT GTA C-3′ 5′-TGA AGT TGC AGG CGA GCA-3′ 5′-CCA CCC TGC TGC TGC TTC TCA TTG TCT-3′
Prostaglandin E2 receptor type 3 (EP3)	Forward Reverse Probe	5′-GAC GGC CAT TCA GCT TAT GG-3′ 5′-TTG AAG ATC ATT TTC AAC ATC ATT ATC A-3′ 5′-CTG TCG GTC TGC TGG TCT CCG CTC-3′
Prostaglandin E2 receptor type 4 (EP4)	Forward Reverse Probe	5′-ACG CCG CCT ACT CCT ACA TG-3′ 5′-AGA GGA CGG TGG CGA GAA T-3′ 5′-ACG CGG GCT TCA GCT CCT TCC T-3′
Prostaglandin F2α receptor (FP)	Forward Reverse Probe	5′-GCA GCT GCG CTT CTT TCA A-3′ 5′-CAC TGT CAT GAA GAT TAC TGA AAA AAA TAC-3′ 5′-CAC AAC CTG CCA GAC GGA AAA CCG-3′
Thromboxane receptor (TBXR)	Forward Reverse Probe	5′-TGG TGG TGG CCA GCG T-3′ 5′-CGG GTT TCG CAG CAC TGT-3′ 5′-TGC CCC TTC TGG TCT TCA TCG CCC-3′
Prostacyclin receptor (IP)	Forward Reverse Probe	5′-GCC CTC CCC CTC TAC CAA-3′ 5′-TTT TCC AAT AAC TGT GGT TTT TGT G-3′ 5′-CCA AGA GCC AGC CCC CTT TCT GC-3′
Phosphodiesterase 4B	Forward Reverse Probe	5′-CCT TCA GTA GCA CCG GAA TCA-3′ 5′-CAA ACA AAC ACA CAG GCA TGT AGT T-3′ 5′-AGC CTG CAG CCG CTC CAG CC-3′
18S	Forward Reverse Probe	5′-CGT CTA CCA CAT CCA AGG AA-3′ 5′-GCT GGA ATT ACG GGG GCT-3′ 5′-TCG TGG CAC CAG ACT TGC CCT C-3′