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Fold induction of expression of PGE2 receptors (EP1-EP4) in HeLa COX-1 Tet-Off cells as determined by real-time quantitative RT-PCR. COX-1 expression was induced for 24, 48, and 72 h in the presence or absence of the COX enzyme inhibitor indomethacin. Fold induction was determined by dividing the relative expression in induced cells by the relative expression in uninduced cells.
