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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Cancer Res. 2002 Jan 15;62(2):424–432.

Fig. 7.

Fig. 7

Western blot analysis of 20 μg of total clarified cell lysate isolated from HeLa COX-1 Tet-Off cells grown for 72 h in the presence of DOX or for 24, 48, and 72 h in the absence of DOX to induce COX-1 expression. A, immunoblot of bFGF expression after DOX withdrawal from the culture medium. bFGF expression was induced coincident with COX-1 overexpression. Up-regulated bFGF expression was abolished by indomethacin and partially inhibited by NS-398. B, immunoblot of VEGF expression after DOX withdrawal from the culture medium. VEGF was induced after 72 h of COX-1 overexpression. Up-regulated VEGF expression was abolished by indomethacin and partially inhibited by NS-398. C, immunoblot of Ang-1 expression after DOX withdrawal from the culture medium. Ang-1 was induced coincident with COX-1 overexpression after 48 h. Up-regulated Ang-1 expression was abolished by indomethacin and partially inhibited by NS-398. D, immunoblot of Ang-2 expression after DOX withdrawal from the culture medium. Ang-1 was induced after 48 h of COX-1 overexpression. Up-regulated Ang-2 expression was abolished by indomethacin and partially inhibited by NS-398. Proteins were normalized for loading against β-actin.