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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Endocrinology. 2008 Mar 27;149(7):3470–3477. doi: 10.1210/en.2007-1695

Fig. 3.

Fig. 3

Colocalization of PKR1 with cytokeratin, CD31, and CD68 in third-trimester human placenta (n = 4). PKR1 was colocalized with cytokeratin 18 (trophoblast marker), CD31 (blood vessel marker), CD68 (macrophage marker), CD3 (T-cell marker), and CD 20 (B-cell marker) by dual-immunofluorescence immunohistochemistry in third-trimester placenta. PKR1 (red, A and D) and cytokeratin 18 (green, B and E) were colocalized (yellow channel, merged, C and F) in syncytiotrophoblasts (st) and in some cytotrophoblasts (ct). PKR1 (red, G) and CD31 (green, H) were colocalized in blood vessel (bv) endothelium within villi (yellow channel, merged, I). PKR1 (red, J) colocalized with CD68 (green, K) to macrophages (m, yellow channel, merged, L). PKR1 did not colocalize with CD3 (M, merged) or CD20 (N, merged). Control sections were incubated with normal IgG from the same host species and displayed no immunoreactivity (O). Original magnification, ×40.