TABLE 1.

The differential lipolytic proteome of mouse adipose tissue

No.		Name	NCBI No.	MW (kDa)	pI	Identified Peptides	Sequence Coverage (%)	Relative Ratios
a		Fatty acid synthase	28461372	242.4	6.13	80	49	−1.6
b		Oxoglutarate dehydrogenase
	1	Oxoglutarate dehydrogenase	15489120	116.5	6.36	48	55	3.7
c		Hormone sensitive lipase
	1	Hormone sensitive lipase	26325924	88.0	8.15	9	17	1.5
d		Esterase 1, Albumin 1
	1	Esterase1	6679689	61.1	5.02	7	17	−2.1
	2	Albumin1	74137565	68.7	5.79	27	58	−1.6
e		Albumin1, Leukotriene-A4 hydrolase, Carnitine palmitoyltransferase 2
	1	Albumin1	74137565	68.7	5.79	40	71	−1.9
	2	Leukotriene-A4 hydrolase	74148023	69.1	5.98	7	14	2.1
	3	Carnitine palmitoyltransferase 2	74139371	74.0	8.59	14	20	1.4
f		Esterase 31-like, Carnitine acetyltransferase
	1	Esterase 31-like	21362301	63.0	5.65	4	7	−7.3
	2	Carnitine acetyltransferase	59802828	70.9	8.53	6	12	−1.6
g		Epoxide Hydrolase, Adipose triglyceride lipase, Carboxylesterase ML1, Triacylglycerol lipase, 3-Oxoacid CoA transferase 1
	1	Epoxide Hydrolase	563510	62.5	5.86	3	8	−1.3
	2	Adipose triglyceride lipase	26327465	53.7	6.06	6	17	−1.3
	3	Carboxylesterase ML1	15488664	61.6	6.03	15	35	−1.4
	4	Triacylglycerol lipase	16716505	61.9	6.30	15	36	−1.4
	5	3-Oxoacid CoA transferase 1	74222276	56.0	8.73	11	29	1.0
h		Enolase 1
	1	Enolase1	53734652	50.2	8.01	19	57	1.6
i		Apolipoprotein A4, S-adenosylhomocysteine hydrolase, CGI-58 like (Abhd5), Fumaryl acetoacetase, Acyl-CoA thioesterase 2
	1	Apolipoprotein A4	38198665	45.0	5.33	12	29	−1.3
	2	S-adenosylhomocysteine hydrolase	74185262	47.8	6.03	8	22	−1.5
	3	CGI-58 like (Abhd 5)	22477988	39.2	6.33	3	10	−1.7
	4	Fumaryl acetoacetase	14789698	46.1	6.92	3	9	−1.8
	5	Acyl-CoA Thioesterase 2	31980998	50.6	8.74	5	12	−1.4
j		Mitochondrial Acyl-CoA thioesterase 1, 3-Ketoacyl-CoA Thiolase, Acetyl-CoA acyltransferase 1, Acetyl-CoA acyltransferase 2
	1	Mitochondrial Acyl-CoA thioesterase 1	74196709	49.7	6.88	15	34	4.5
	2	3-Ketoacyl-CoA thiolase	29126205	41.8	8.33	14	55	3.2
	3	Mitochondrial acetoacetyl-CoA thiolase	19354555	44.8	8.71	7	23	5.8
k		Acetyl-CoA acetyltransferase 3, Acetyl-CoA acetyltransferase 2
	1	Acetyl-CoA acetyltransferase 3	29612600	41.5	8.09	2	12	4.2
	2	Acetyl-CoA acetyltransferase 2	30348956	41.3	6.60	4	13	1.1
l		Esterase D, Monoglyceride lipase, Williams-Beuren syndrome critical region
	1	Esterase D	12846304	34.9	8.51	7	37	−1.6
	2	Monoglyceride lipase	74201613	37.5	8.91	7	24	−3.1
m		Esterase 1 homolog, Glutathione S-transferase μ2, Glutathione S-transferase θ2, Glutathione S-transferase ζ1
	1	Esterase 1 homolog	20070420	28.1	9.00	9	43	1.3
	2	Glutathione S-transferase μ2	22477996	25.7	6.91	14	64	1.4
	3	Glutathione-S-transferase θ2	2495110	27.7	7.03	5	27	−1.4
	4	Glutathione-S-transferase ζ1	21594192	24.3	7.68	3	18	1.8
n		Lysophospholipase 1, Lysophospholipase 2
	1	Lysophospholipase 1	15488808	24.7	6.14	4	20	3.3
	2	Lysophospholipase 2	45768815	24.8	6.74	3	10	2.2

Letters and numbers correspond to the fluorescent protein lanes (1D PAGE) and spots (2D PAGE) in Fig. 5. All proteins in this list were identified by nano-HPLC-MS/MS analysis. The relative ratios were calculated using the formula indicated in Results. Active enzymes are considered more abundant if relative ratios are lower than −1.9 (WAT) and higher than −1.9 (BAT).