Fig. 5.

Distribution of PCPE2 in human plasma. Human plasma was fractionated using Superose 6 columns as described under Materials and Methods. Cholesterol and apoAI were determined and plotted as a function of column fraction. The position at which known lipoproteins eluted from the column is indicated. The presence of PCPE2 was determined by immunoprecipitation, SDS-PAGE, and immunoblotting. Molecular weigh standards and recombinant PCPE2 were included to confirm the location of PCPE2. The figure shows a representative elution profile from three independent experiments.