TABLE 3.
Relative lipid composition of nascent VLDL particles upon a 3-h basal infusion or hyperinsulinemic euglycemic clamp in fasted mice fed a control or T0901317-supplemented diet for one week
| Control |
T0901317 |
|||
|---|---|---|---|---|
| Basal | Clamp | Basal | Clamp | |
| Free cholesterol (%) | 11.4 ± 1.2a,b | 14.1 ± 1.4a,b | 10.4 ± 0.8a | 10.8 ± 0.6a |
| Cholesteryl esters (%) | 1.0 ± 0.4 | 2.5 ± 1.0 | 0.9 ± 0.5 | 1.6 ± 1.0 |
| Phospholipids (%) | 13.1 ± 0.6a | 14.3 ± 0.7a | 11.2 ± 0.5b | 11.5 ± 0.8b |
| Triacylglycerol (%) | 74.6 ± 1.5a | 69.0 ± 2.2 | 77.5 ± 1.9a | 76.2 ± 1.1a |
| Surface to core ratio | 0.324 ± 0.029a,b | 0.398 ± 0.039a,b | 0.275 ± 0.020a | 0.286 ± 0.14a |
| Calculated particle size (nm) | 88.7 ± 4.9a | 77.6 ± 4.4 | 104.2 ± 6.1b | 100.6 ± 6.2a,b |
VLDL particles were isolated by ultracentrifugation from plasma retrieved 1.5 h after Triton WR-1339 injection. The percents of lipids are the molar percents of the total lipids in the VLDL. The surface-to-core ratio is the molar ratio free cholesterol and phospholipids versus triacylglycerol and cholesteryl esters. The particle size is calculated using the equation in which the diameter (nm) = (60 × {[0.211 x (mol of triacylglycerol)/(mol of phospholipids)] + 0.27)}). Values are averages ± SD; n = 5; values which do not share a superscript symbol are significantly different.