TABLE 1.
Physical properties of apoA-I variants
| ApoA-I Variants | Lipid-Free α-Helicity (%)a | Lipid-Bound α-Helicity (%)b | ANS Fluorescence Intensityc |
|---|---|---|---|
| WT | 44 ± 4 | 70 ± 4 | 1.0 |
| R173K | 44 ± 3 | 74 ± 1 | 1.1 |
| R173S | 44 ± 1 | 72 ± 3 | 1.2 |
| Milano (monomer) | 42 ± 4 | 65 ± 8 | 1.6 |
| Milano (dimer) | 33 ± 2d | 45 ± 6d | 1.6 |
a
Mean ± SD from at least four independent measurements.
b
Mean ± SD from three independent measurements. ApoA-I bound to egg PC SUV at a 60:1 (PC:apoA-I) molar ratio.
c
Values are ratios to WT apoA-I. Error is within 0.1.
d
P < 0.01 compared with WT apoA-I.