TABLE 3.
DMPC clearance rate and ITC parameters of SUV binding of apoA-I variants
| ApoA-I Variants | DMPC Clearance Ratea | ΔH (kcal/mol)b | Decay rateb,c |
|---|---|---|---|
| WT | 1.0 | −92.6 ± 5.3 | 1.0 |
| R173K | 1.2 | −94.7 ± 5.7 | 1.0 |
| R173S | 1.1 | −88.1 ± 5.8d | 1.5 |
| Milano (monomer) | 1.0 | −70.6 ± 5.5d | 2.2 |
| Milano (dimer) | 0.5 | −64.2 ± 3.7d | 2.7 |
a
Relative rate of DMPC clearance compared with WT apoA-I. Average of 8 to 12 runs per sample.
b
Parameters of egg PC SUV binding from ITC, mean ± SD (n = 4–6).
c
Values are ratios to WT apoA-I; estimated error is within ± 0.05. The rate constant for WT apoA-I was 1.19 ± 0.03 min−1.
d
P < 0.01 compared with WT apoA-I.