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. 2009 Jun 26;5(6):e1000535. doi: 10.1371/journal.pgen.1000535

Figure 6. Reintroduction of telomerase activity restores telomere pattern and life span of the type II survivors.

Figure 6

Southern blot analysis showing the dynamic changes of telomeric DNA in type II survivors after being transformed with EST2 (wild-type), catalytically inactive est2 (DD670-1AA), or the vector control plasmid. Cells were serially restreaked and genomic DNA from cells obtained after each indicated restreaks (labeled on top of each panel) was digested with restriction enzyme and probed with a telomere probe. (A) Genomic DNA was digested with XhoI. (B) Genomic DNA was digested with 4 bp cutter (MspI, HaeIII, HinfI, AluI). (C) Life span analysis of the 1st restreaked cells after plasmid transformation. Mean life span of strains shown: BY4742+Vector, 23.3±9.5 (n = 56); est2Δ type II+EST2, 24.6±10.1 (n = 58); est2Δ type II+vector, 12.1±4.9 (n = 59); est2Δ type II+est2 (DD670-1AA), 12.1±4.1 (n = 60). (D) Life span analysis of strains freshly transformed with EST3 (wild-type) or the vector control plasmid. Mean life span of strains shown: BY4742+Vector, 29.4±12.8 (n = 60); est3Δ type II+EST3, 29.8±11.1 (n = 60).