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. 2009 Jun 19;5(6):e1000488. doi: 10.1371/journal.ppat.1000488

Figure 5. HDAC1 silences defense gene expression and facilitates A. phagocytophilum infection of THP-1 cells.

Figure 5

(A) THP-1 cells were transfected with HDAC1 or HDAC2 siRNA and were infected 24 hours after transfection with cell-free A. phagocytophilum. The A. phagocytophilum load was determined 24 h after infection by qPCR and normalized to infection levels in non-transfected cells. (B) THP-1 cells were transfected with HDAC1 siRNA for silencing or with pHDAC1-FLAG plasmid to overexpress HDAC1 and were infected 24 hours after transfection with cell-free GFP-expressing HGE-1 strain of A. phagocytophilum. The percent of infected cells was determined 24 h after infection by flow cytometry and normalized to infection levels in untreated cells. (C) Expression of defense genes by THP-1 cells infected with A. phagocytophilum–infected or transfected with pHDAC1-FLAG plasmid or HDAC1 siRNA was determined by qRT-PCR. Gene expression was normalized to housekeeping genes and to the level of expression of untreated, uninfected THP-1 cells.