Fig. 4.

Methylation analysis of the RUNX3 gene in cells. MSP results were available for 10 cell lines (except SCC-1395, Hs 578T, and T-47D). Methylated DNA was found in 6 cell lines (MCF7, SNU-1, MDA-MB-231, SK-BR-3, MCF-7 TH, and MCF 10A), and unmethylated DNA in 5 (MDA-MB-436, MDA-MB-468, SK-BR-3, ZR-75-1, and SNU-5). U and M denote unmethylation and methylation, respectively. N denotes normal human genomic DNA, which was used as an unmethylated PCR control, and DW is an abbreviation for distilled water, which was used as a negative PCR control.