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. 2009 Apr 7;100(9):1415–1424. doi: 10.1038/sj.bjc.6605021

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Copyright © 2009 Cancer Research UK

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Long JNK1 isoforms have a proapoptotic function. (A) Knockdown of JNK1α2/β2 in Colo205 cells by siRNA. Cells were nucleofected with two different JNK1α2/β2 siRNAs, or an siRNA against GFP as a negative control. Cell lysates were prepared 24 h after transfection and JNK1-short and -long expression was detected with primers western blotting. (B) Knockdown of JNK1α2/β2 reduces TRAIL-mediated apoptosis. Colo205 cells were treated with rhTRAIL (40 and 60 ng ml⁻¹) for 3 h, after which percentage of apoptotic cells was determined with Annexin V. The graph shows the average cell death induced±s.e.m. of three independent experiments (sum of Ann V⁺/PI⁻ and Ann V⁺/PI⁺ percentages). The asterisk (^*) indicates significant differences (P<0.05).