Table 4.
Peptides identified by nano-LC-MS/MS in each gel band of histone H2A**
| Gel band | Observed (m/z) | Mr, Da (expt) | Mr, Da (calc) | Delta | Miss* | Peptide*** |
|---|---|---|---|---|---|---|
| H2A_0 | 419.442+ | 836.87 | 836.41 | 0.46 | 1 | 36KGNYAER42 |
| 426.242+ | 850.47 | 849.52 | 0.96 | 0 | 82HLQLAIR88 | |
| 861.31+ | 860.30 | 860.39 | −0.08 | 0 | 89NDEELNK95 | |
| 431.322+ | 860.62 | 860.39 | 0.24 | 0 | 89NDEELNK95 | |
| 944.50+ | 943.49 | 943.52 | −0.03 | 0 | 21AGLQFPVGR29 | |
| 473.072+ | 944.13 | 943.52 | 0.61 | 0 | 21AGLQFPVGR29 | |
| 637.222+ | 1272.43 | 1271.67 | 0.76 | 1 | 89NDEELNKLLGK99 | |
| 651.122+ | 1300.23 | 1299.70 | 0.53 | 1 | 89NDEELNKLLGR99 | |
| 966.352+ | 1930.69 | 1930.16 | 0.53 | 0 | 100VTIAQGGVLPNIQAVLLPK118 | |
| H2A_1 | 430.33+ | 429.32 | 429.30 | 0.03 | 0 | 96LLGK99 |
| 498.49+ | 497.48 | 497.33 | 0.15 | 0 | 78IIPR81 | |
| 355.882+ | 709.75 | 708.32 | 1.43 | 0 | 37GNYAER42 | |
| 419.592+ | 837.17 | 836.41 | 0.76 | 1 | 36KGNYAER42 | |
| 426.242+ | 850.47 | 849.52 | 0.96 | 0 | 82HLQLAIR88 | |
| 431.622+ | 861.23 | 860.39 | 0.85 | 0 | 89NDEELNK95 | |
| 473.262+ | 944.51 | 943.52 | 0.99 | 0 | 21AGLQFPVGR29 | |
| 637.002+ | 1271.99 | 1271.67 | 0.32 | 1 | 89NDEELNKLLGK99 | |
| 651.122+ | 1300.22 | 1299.70 | 0.52 | 1 | 89NDEELNKLLGR99 | |
| 847.232+ | 1692.44 | 1691.90 | 0.55 | 1 | 82HLQLAIRNDEELNK95 | |
| 966.322+ | 1930.63 | 1930.16 | 0.47 | 0 | 100VTIAQGGVLPNIQAVLLPK118 | |
| 1931.94+ | 1930.93 | 1930.16 | 0.77 | 0 | 100VTIAQGGVLPNIQAVLLPK118 | |
| 1136.592+ | 2271.17 | 2270.37 | 0.79 | 0 | 96LLGGVTIAQGGVLPNIQAVLLPK118 | |
| 434.212+ | 866.41 | 865.44 | 0.97 | 1 | 119KTESHHK125 | |
| 427.452+ | 852.89 | 852.41 | 0.48 | 1 | 36KGNYSER42 | |
| H2A_2 | 498.46+ | 497.45 | 497.33 | 0.12 | 0 | 78IIPR81 |
| 355.572+ | 709.12 | 708.32 | 0.80 | 0 | 37GNYAER42 | |
| 419.802+ | 837.58 | 836.41 | 1.17 | 1 | 36KGNYAER42 | |
| 850.45+ | 849.44 | 849.52 | −0.08 | 0 | 82HLQLAIR88 | |
| 426.382+ | 850.75 | 849.52 | 1.24 | 0 | 82HLQLAIR88 | |
| 426.262+ | 850.51 | 849.52 | 0.99 | 0 | 82HLQLAVR(Me)88 | |
| 861.38+ | 860.37 | 860.39 | −0.01 | 0 | 89NDEELNK95 | |
| 431.602+ | 861.18 | 860.39 | 0.80 | 0 | 89NDEELNK95 | |
| 944.51+ | 943.50 | 943.52 | −0.02 | 0 | 21AGLQFPVGR29 | |
| 472.962+ | 943.90 | 943.52 | 0.38 | 0 | 21AGLQFPVGR29 | |
| 637.242+ | 1272.47 | 1271.67 | 0.80 | 1 | 89NDEELNKLLGK99 | |
| 650.972+ | 1299.92 | 1299.70 | 0.22 | 1 | 89NDEELNKLLGR99 | |
| 658.722+ | 1315.43 | 1313.68 | 1.75 | 1 | 89NDEELNK(Ac)LLGK99 | |
| 672.672+ | 1343.32 | 1341.71 | 1.61 | 1 | 89NDEELNK(Ac)LLGR99 | |
| 1930.982+ | 1929.97 | 1930.16 | −0.19 | 0 | 100VTIAQGGVLPNIQAVLLPK118 | |
| 966.552+ | 1931.08 | 1930.16 | 0.92 | 0 | 100VTIAQGGVLPNIQAVLLPK118 | |
| 1136.392+ | 2270.77 | 2270.37 | 0.39 | 0 | 96LLGGVTIAQGGVLPNIQAVLLPK118 | |
| 419.122+ | 836.23 | 835.50 | 0.73 | 0 | 82HLQLAVR88 |
*
Miss: the # of miscleavage sites;
**
The protein abundance in band 3 was too low to be detected by MS.
***
Any peptide hit with Mascot Score < 18 was not considered as a match.