Fig. (2). takeout expression requires both doublesex and fruitless.

(A, B) Northern analysis of takeout expression and cartoon interpretation of takeout transcriptional regulation in males (A) and females (B). Note that while both DSX-M and FRU^M are required there is no evidence that they bind directly or interact physically with each other. (A) takeout expression in dsx¹ (lane 2) and fru⁴/fru³ mutant males is reduced (lane 4), when compared to their heterozygous siblings (lanes 1 and 3). (B) Forced expression of male-specific forms of dsx and fru act synergistically to induce takeout in XX individuals. Females expressing the male DSX-M from the dominant mutation dsx^SWE show activation of takeout (lane 6) compared to control females (lane 2). These females are dsx^SWE/ dsx⁺ and produce both dsx^F and dsx^M. There is no effect of dsx^SWE/ dsx⁺ on takeout expression in males (compare lanes 5 and 1). No significant induction of takeout was observed in chromosomal females expressing fru in fat body (lane 4). Females expressing both fru and dsx^SWE (lane 8) have takeout expression levels indistinguishable from their male siblings (lane 7). takeout expression in wildtype males and females is shown in lane 1 and 2 (Modified with permission from Dauwalder et al. Genes & Dev., Nov 2002; 16: 2879 – 2892, Copyright 2002, Cold Spring Harbor Laboratory Press).