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. 2009 Jun 23;3(6):e464. doi: 10.1371/journal.pntd.0000464

Figure 1. PZQ miscues regeneration to produce two-headed flatworms.

Figure 1

(A) Schematic of regenerative assay. Worms were exposed to drugs during trunk fragment isolation (dashed line, mins) and for variable times thereafter (solid bar, days). Anterior-Posterior (AP) polarity was scored at least 7 days after cutting. (B) Two headed flatworm that regenerated from a trunk fragment exposed to PZQ (70 µM, 48 hrs). (C) Regenerative timecourse. Following isolation of trunk fragment (boxed), exposure to PZQ resulted in the appearance of eyespots by 3 to 3.5 days (red arrows), and a second pharynx by 5 to 6 days (green arrows). (D) Dose dependent effect of PZQ on bipolar regeneration (note 100% = all worms regenerated two heads; data is not normalized). (E) Duration and timing of PZQ exposure impacted bipolarity. Different plots represent exposure to PZQ (70 µM) offset at different times (square, black triangle, white triangle; 6, 12 & 18 hrs, respectively) after cutting and for the indicated duration (x-axis).