Fig. (1).

Dose-dependence of hydrogen peroxide PMCA protein levels. (A) PMCA1, 130 kDa (B) PMCA2, 120 kDa (C) PMCA3, 134 kDa (D) PMCA4 129 and 133 kDa. (Top Inset) A typical Western Blot showing dose-dependent effects of hydrogen peroxide on PMCA protein expression in the HLE B-3 cells treated for 4 hours. An equal amount of membrane protein was loaded in each lane and subjected to electrophoresis. Blots were incubated with a specific PMCA antibody, then stripped and reprobed with β-actin antibody (lower Western Blot insert, 42 kDa). (Bars) Densitometric analysis of Western Blots. Results are presented as mean ± standard error of 3 or 4 separate experiments. A value of P ≤ 0.05 was considered significant (*).