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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Sci Signal. 2008 Nov 25;1(47):ra15. doi: 10.1126/scisignal.1164263

Figure 5. Identification of a subset of LPS-inducible genes that are repressed by the LRP1 ICD.

Figure 5

A) Total RNA from wildtype and LRP1-deficient MEF and from LRP1-deficient fibroblasts stably re-transfected with LRP1, the membrane-bound LRP1 ICD (LRP-β-chain), the LRP1 ECD, the free LRP1 ICD (LRP1-105) or the empty plasmid vector was analyzed by quantitative real-time PCR for expression levels of Usp18, Rsad2, Tyki, and Oas1. Bars represent the mean of 6 independent experiments, error bars depict SEM. Statistical analysis was done using the Student’s t-test. * p<0.001.

B) Wildtype fibroblasts were treated with 2 µg/ml LPS for 3h or were left untreated. RNA was prepared and examined by real-time PCR for expression of Usp18, Rsad2, Tyki, and Oas1. ** p<0.05, n=5.