A) RNA samples from wildtype and LRP1-deficient MEF were analyzed by Northern Blotting with cDNA probes for the genes indicated. Cyclophilin served as a loading control. A representative experiment of n=3 is shown.
Dkk-3 – dickkopf-3, C3 – complement factor 3, sfrp-1 – secreted frizzled-related protein-1, AHR – aryl hydrocarbon receptor.
B) RNA samples from wildtype and LRP1-deficient MEF and from LRP1-deficient fibroblasts stably re-transfected with an expression plasmid for LRP1 (control: empty plasmid vector) were examined for 25-cholesterol-hydroxylase expression. A representative experiment of n=3 is shown.