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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: J Proteome Res. 2008 May 30;7(7):2812–2824. doi: 10.1021/pr700816k

Figure 1. Pro-Q Diamond phosphoenrichment resin selectively binds phosphorylated protein.

Figure 1

Bcr-Abl kinase was incubated with GST- SH3n-Abltide and [γ-32P]-ATP to allow for phosphorylation of GST- SH3n-Abltide. The solution was passed over a column containing Pro-Q Diamond resin and samples were taken from the loaded sample (L), flow-through (FT), elution (E) and remaining beads after elution (Beads). (a) Each fraction was subjected to SDS-PAGE and the phosphoprotein detected using a phosphorimager. The two E lanes represent the same sample loaded twice, first a 5uL aliquot of the sample (1X) and then with a 4-fold higher amount of sample or 20 uL (4X). (b) Total phosphorylation in each sample after accounting for sample dilution and loading. The star (*) represents that only a small sample (3%) of the loaded sample (L) was loaded on the gel. The remainder of sample (L) was used for the Pro-Q phosphoenrichment column and is represented in lanes FT, E, E2 and Beads.