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. 2009 Jun 1;3:5. doi: 10.3389/neuro.05.005.2009

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Copyright © 2009 Jakovcevski, Filipovic, Mo, Rakic and Zecevic.

This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.

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Cell death in chronic slice cultures. (A) The representative image of activated caspase-3 staining (red, arrows) on the ventricular/subventricular zone (VZ/SVZ) of the slice culture from a 19-week-old human fetus after 5 days in vitro. Nuclei are counterstained with bisbenzamide (blue). Scale bar: 20 μm. (B) Proportion of caspase-3a⁺ cells in all bisbenzamide-labeled nuclei in the VZ/SVZ and intermediate zone/cortical plate (IZ/CP) of 19-week-old human fetus. Note the higher percentage of apoptotic cells in the VZ/SVZ, similar to results obtained by TUNEL staining on frozen sections (Rakic and Zecevic, 2000).