Figure 3.

Cells of oligodendrocyte lineage in culture. (A) Early oligodendrocyte precursors expressing PDGFRα. (B) O4⁺ late oligodendrocyte precursors (green), some of them double-labeled with NG2 (yellow in overlay) and some early oligodendrocyte precursors labeled only with NG2 (red). (C) GFAP⁺ astrocyte (green) among MBP⁺ oligodendrocytes (red). (D) All cultured oligodendrocytes express nuclear Olig2 (red), here shown in the MBP⁺ mature oligodendrocytes (green). (E, F) Confocal image of an oligodendrocyte with Olig2⁺ nucleus that produces MBP⁺ myelin-like membrane (green). Interestingly, Olig2 expression is not localized only to the cell nucleus, but it is also seen in the cytoplasm. Scale bar: (A–D) 20 μm, (E, F) 10 μm.