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. 2009 May 19;5:270. doi: 10.1038/msb.2009.28

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Copyright © 2009, EMBO and Nature Publishing Group

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits distribution and reproduction in any medium, provided the original author and source are credited. Creation of derivative works is permitted but the resulting work may be distributed only under the same or similar licence to this one. This licence does not permit commercial exploitation without specific permission.

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Cellular localization of mutagenized PhoB^D53A-GFP in roots of transgenic Arabidopsis plants. (A) Fluorescence from PhoB^D53A-GFP is diffused in an untreated root. (B) The same root showing PhoB^D53A-GFP localization after cytokinin treatment. (C, D) Detailed view of a root showing that nuclear localization of PhoB^D53A-GFP is variable and sporadic (arrowheads point to nuclei). (E, F) Detail view of another root showing that PhoB^D53A-GFP accumulates at the base of cortical cells (arrows). Some nuclear localization of PhoB^D53A-GFP can be seen in the root vascular tissue (arrowheads). −CK, tissues before cytokinin treatment; +CK same tissue after cytokinin treatment; DAPI, tissues treated with DAPI to stain DNA. Scale bars, 50 μm in (A, B); scale bars, 10 μm in (C–F).