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. 2009 Apr 6;18(13):2344–2358. doi: 10.1093/hmg/ddp167

Figure 2.

Figure 2.

Localization of TRIM32 and dysbindin in skeletal muscle. Longitudinal sections of guinea pig tibialis anterior muscle were stained with the antibodies 3293 (TRIM32), EA-53 (α-actinin), B4 (myomesin) and PA3111A (dysbindin). Primary antibodies were detected using species-specific fluorescent antibody conjugates (Alexa-488 or rhodamine Red-X). TRIM32 and dysbindin colocalize with α-actinin, suggesting that each protein is found on and around the periphery of the Z-line. In addition to the Z-line labelling, dysbindin is also found at the sarcolemma and M-band. Pre-incubation of the anti-TRIM32 antibody with the fusion protein used for immunization blocked labelling in muscle sections but did not interfere with immunoreactivity of α-actinin on the same tissue section. Scale bar is 20 µm.