Figure 7.

Impaired monoubiquitination of D487N. HEK293T cells were transfected with myc-tagged constructs encoding, TRIM32, TRIM32–D487N, TRIM32–RING-mut and TRIM32–P130S as indicated. After 24 h, each protein was immunoprecipitated from cell extracts using the 9E10 antibody. Immune complexes were separated by gel electrophoresis on a 4–20% gradient gel. The gel was stained with colloidal Coomassie blue to visualize protein bands. Bands A1 and A3 were excised and subjected to FTICR-MS. The table shows the percentage of their amino acid sequence covered by peptides identified in MS and the number of unique peptides that match this sequence.