Figure 3.

Experimental design for experiment I. Group 1 is the surgical oocyte retrieval (SOR) group. Groups 2 and 3 are control groups and were superovulated but did not undergo surgery. (A) The experimental design to compare fertilization and embryo transfer successes is shown. In this experiment, SOR was not performed on the right oviduct. (B) The experimental design to determine the maximal number of oocytes that can be collected from a mouse is shown. SOR was performed on the left and right oviducts. *, One mouse did not ovulate and was excluded from the second collection. IVF, in vitro fertilization; ET, embryo transfer.