The anesthetic used during SOR affects CByB6F1/J oocytes. The percentages of live, dead, and fragmented oocytes after SOR using tribromoethanol or isoflurane anesthesia are shown. Mice were superovulated and anesthetized 14 to 15 h after hCG injection. Cumulus oocyte masses were collected, and oocytes were dissociated for counting using hyaluronidase. Values are mean ± SEM, n = 5 per group. Using tribromoethanol as an anesthetic resulted in fewer (*, P < 0.05) live oocytes and more (+, P < 0.05) dead oocytes than those from the isoflurane and control groups.