Table 1.
Using tissue adhesive to seal the ampulla incision increases the number of oocytes retrieved at the second collection
| No. of oocytes after SOR | No. of oocytes at 2nd collection |
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| No. of mice | Left ovary | Right ovary | ||
| No tissue adhesive | 5 | 9 ± 1 | 0b | 10 ± 2a |
| Tissue adhesive | 15 | 10 ± 2 | 3 ± 1a,b | 10 ± 1a |
| Sham surgery | 5 | not done | 6 ± 1a | 4 ± 1b |
CByB6F1/J mice were superovulated, and oocytes were collected by SOR from the left oviduct only. At 2 to 6 wk after SOR, oocytes were collected from the left and right oviducts after euthanasia. Results did not differ between the week 2 and 6 collections (data not shown), therefore these data were combined. The ampulla incision was either closed with tissue adhesive or not closed. Sham surgery was identical to SOR except that no ampulla incision was made. Data are presented as mean ± SEM.
a,b
Values with different superscripts within the same column are different (P < 0.05).