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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Dev Biol. 2007 Mar 24;306(1):241–254. doi: 10.1016/j.ydbio.2007.03.024

Figure 1.

Figure 1

Effect of Fertilization on the distribution of P-Tyr-containing egg proteins. MII oocytes were recovered from the oviducts of superovulated mice at 14 hrs post-hCG and fertilizing eggs and zygotes were collected from superovulated mated females at 13.0–16.0 hrs post hCG. The oocytes or zygotes were dissected free of the oviduct in the presence of fixative and processed for immunofluorescence as described in “Materials and Methods”. The samples were stained with the mouse anti-P-Tyr monoclonal 4G10 followed by Alexa-488-goat anti-mouse IgG (green) as well as Hoechst 33258 (white) to detect DNA. In some cases, the spindle was stained with rat monoclonal YOL 1/34 anti-tubulin which was detected by Alexa-568-goat anti-rat (red). Samples were examined on a Zeiss LSM500 confocal microscope as described in “Methods”. (A), MII oocyte; (B), fertilized, early anaphase; (C) fertilized telophase; (D) fertilized, early pronuclear stage (16 hrs post-hCG). Specificity was demonstrated by incubating eggs in the presence of the 4G10 antibody + 1mM P-Tyr (E). Magnification is indicated by the bars which represent 10μm.